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The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products have been digested and cloned into a pET21a+ vector working with NdeI and XhoI restriction enzymes. A point mutation was released by the fast adjust site-directed mutagenesis method ut